• The GTM Guild
  • Posts
  • A/B Testing Cold Emails: What to Test and Why It Matters

A/B Testing Cold Emails: What to Test and Why It Matters

The small tweaks that change your entire reply rate

In partnership with

Cold email isn’t guesswork. It’s an ongoing experiment. The most effective teams don’t just “send and hope”—they test, learn, and refine.

A/B testing gives you insight into what actually drives opens, clicks, and most importantly—replies.

Whether you're a founder writing emails manually or a sales team running sequences at scale, this issue breaks down:

  • What to test

  • Why it matters

  • How to do it right without overcomplicating your process

The Website Team Trusted by the Highest Performing Marketers

The perfect website team is reasonably priced, incredibly good at communication, highly responsive, very transparent, and actually cares about your success. If your current team falls short on any of that, we would love a chance to prove ourselves.

Most marketers have resources but rarely a true expert partner for design, development, or website management. That is where we come in. OneNine becomes your website team for as many or as few tasks as you need.

Whether you need just design, just development, or both, every OneNine client gets a dedicated project manager who keeps projects moving quickly without needing your constant oversight. We only do website design, development, and management. There are many agencies, but few can say they have been doing this for more than 13 years with a fully North American team and hundreds of active clients who trust them. We can.

Why A/B Testing Is Essential for Outbound

Most cold email campaigns fail quietly. Not because the idea is wrong, but because the message doesn’t land.

A/B testing helps you:

  • Identify what resonates with your audience

  • Optimize subject lines and calls to action

  • Improve response rates over time

  • Build a repeatable system that scales

The difference between a 1% and a 10% reply rate often comes down to small changes made intentionally.

What You Should Be Testing

Here are 6 elements worth A/B testing in your cold emails:

1. Subject Line

This decides whether the email gets opened at all.

Test examples:

  • Question vs. statement

  • Short (1–3 words) vs. longer subject lines

  • Personalized vs. generic

What to look for:
Open rates and reply rates. A high open rate with no replies means your subject line worked, but your message didn’t.

2. First Line or Hook

The first line shows up in the inbox preview. It’s your second chance to earn a read.

Test examples:

  • Personal line (“Saw your recent post…”)

  • Value hook (“I help GTM teams reduce churn by X%”)

What to look for:
Does a personalized opening perform better than a straight-to-the-point one?

3. Email Length

Some audiences prefer quick, direct messages. Others need a bit more context.

Test examples:

  • 3-sentence message vs. 7-sentence message

  • Bullet point format vs. short paragraph

What to look for:
How far people scroll, how often they reply.

4. Call to Action (CTA)

This is what you want them to do next.

Test examples:

  • “Are you the right person to speak to?”

  • “Interested in a quick 15-min call next week?”

  • “Can I send over more details?”

What to look for:
Which CTA gets the most positive replies, not just clicks.

5. Tone and Language

Tone creates trust. Test formal vs. casual. Sharp vs. friendly.

Test examples:

  • “We noticed…” vs. “I came across your work…”

  • “Let me know if this is relevant” vs. “No pressure if not a fit”

What to look for:
Which voice sounds more human and earns a reply?

6. Signature and Sender Info

People respond to people—not just brands.

Test examples:

  • Full name + title vs. just first name

  • Adding LinkedIn link vs. plain text signature

What to look for:
Are people more likely to trust a peer than a salesperson?

How to Run A/B Tests Without Overcomplicating

  • Test one variable at a time. If you change too many things, you won’t know what worked.

  • Send at least 100 emails per variation. Anything less can give you false signals.

  • Track outcomes, not just opens. Your goal is replies and qualified leads—not vanity metrics.

  • Use your learnings across channels. High-performing subject lines often work as DM openers or ad copy too.

Common Pitfalls to Avoid

  • Testing too soon without volume

  • Drawing conclusions from too little data

  • Not documenting what you learned

  • Testing just to test, without a clear hypothesis

Remember: Testing is only useful if you act on what you learn.

The GTM Guild POV

A/B testing isn’t just for marketers or large-scale sales orgs.
It’s a mindset: every cold email is a chance to learn.

If you treat cold outreach as a living experiment, you’ll find the right message faster—and scale it with confidence.

You don’t need more tools. You need tighter loops between what you try and what you learn.

Until next drop,
– GTM Guild